We routinely use 31P NMR
to study lipid polymorphism. It is
complementary to diffraction,
DSC and
other physical studies and can give
information about highly curved structures
with
no long range order. We are
currently doing solid state NMR to gain
information about the
molecular organization in
membranes. Also, peptide-lipid interactions have been studied in our
lab
with the use of transfer NOEs .
References:
R.M.
Epand, R.F. Epand, A.D. Bain, B.G. Sayer and D.W. Hughes. 2004. Properties
of polyunsaturated
phosphatidylcholine membranes in the presence and absence
of cholesterol. Magn. Reson. Chem. 42(2) 139-147.
R.F.
Epand , J.C. Martinou, M. Fornallaz-Mulhouser and R.M. Epand. 2002. The
apoptotic protein tBid
promotes leakage by altering membrane curvature. J.
Biol. Chem., 277(36):32632-32639.
Z.
Zhou , J.C. Makosko, D.W. Hughes, B.G.
Sayer, J. Hawes and R.M. Epand.
2000.
15N-NMR study
of the ionization properties of the
influenza virus fusion
peptide in zwitterionic
phospholipid dispersions. Biophys. J., 78:2418-2425.
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