†††††††††††††††††††††† Dr RICHARD M. EPAND

††††††††††††††††††††††† Professor, Department of Biochemistry and Biomedical Sciences,

††††††††††††††††††††††††††††††††††††††††††††††††† McMaster University

††††††††††††††††††††††††††††††††††††††††††††††††††††† Hamilton, ON, Canada

 
 
A.B. (Johns Hopkins University, Baltimore, MD
 
Ph.D. (Columbia University, N.Y)
 
Postdoctoral Fellow (Cornell University, N.Y.)
 
Visiting Scientist: Yale University, CT 
††††
††Visiting Scientist: Campomar Research Institute
 
Avanti Award in Lipid from the Biophysical Society, given to an investigator
 
for seminal studies in lipid metabolism, lipid enzymology or membranes.
 
Senior Investigator Award of the Canadian Institutes of Health Research
 
Fellow of the Biophysical Society
 
Honorary Fellow of the Spanish Biophysical Society
 
Fellow of the Biophysical Society of Canada 
 
 
 
 
 
 
 
†††††††††††††††††††††††††††††††††††† epand@mcmaster.ca††††††††††††††††††††††††† 
††††††††††
  

 

††††††††††††††† RELATIONSHIP BETWEEN MEMBRANE PROPERTIES AND BIOLOGICAL FUNCTION

Many cell processes are intimately linked to their membranes. These include signal transduction phenomena, as well as the action 
of many drugs and cytotoxic agents. We are studying the molecular  properties  of  biological membranes and their relationship to biological 
function including the phosphatidylinositol cycle and the mechanisms by which the lipid intermediates of this cycle become enriched in specific 
acyl chains. The first step in this cycle is catalyzed by diacylglycerol kinase (DGK). In mammals there are 10 known isoforms of this enzyme, 
each having a different function and structure. Several disease processes have a connection with changes in expression of one or more of these 
isoforms each of which have specific roles in cells. We are also studying membrane curvature and the role of the acyl transferase enzyme, tafazzin, 
in determining the acyl chain composition of cardiolipin. Mutations in tafazzin lead to an inherited disease, Barth syndrome, in which patients suffer 
from altered cardiolipin and defective mitochondrial function. The roles of mitochondrial membrane curvature properties in processes connected 
to apoptosis is also under investigation. 
 

 


 

RECENT PUBLICATIONS:

 

 

W. Jennings, S. Doshi, K. DíSousa and RM Epand. 2015. Molecular Properties of diacylglycerol kinase-epsilon in

Relation to function. Chem. Phys. Lipids, S0009-3084(15):30001-30003.

 

RM Epand, D. Bach and E. Wachtel. 2015. Comment on ďCholesterol solubility limit in lipid membranes probed by

small angle neutron scattering and MD simulationsĒ by S. Garg et al. Soft Matter 2014, 10, 9313. Soft Matter,

11(27):5580-5581.

 

K. DíSousa and RM Epand. 2015. The phosphatidylinositol synthase-catalyzed formation of phosphatidylinositol does not

exhibit acyl chain specificity. Biochemistry, 54(5):1151-1153.

 

RM Epand. 2015. Introduction to membrane lipids. Methods Mol Biol 1232:1-6.

 

U. Schlattner, M. Tokarska-Schlattner, RM Epand, M Boissan, ML Lacombe, J Klein-Seetharaman, VE Kagan. 2015.

Mitochondrial NM23-H4/NDPK-D: abifunctional nanoswitch for bioenergetics and lipid signaling. Naunyn Schniedebergs Arch Pharmacol, 388(2):271-278.

 

RM Epand, K. DíSouza, B. Berno and M. Schlamme. 2015. Membrane curvature modulation of protein activity determined

by NMR. Biochim. Biophys. Acta, 1848(1 PtB):220-228.

 

K. DíSousa, YJ Kim, T. Balla amd RM Epand. 2014. Distinct properties of the two isoforms of CDP-diacylglycerol kinase.

Biochemistry, 53(47):7358-7367.

 

G. Wang, B. Mishra, RF Epand and RM Epand. 2014. Cathelicidin LL-37 and its fragments. Biochim. Biophys. Acta,

1838(9):2160-2172.

 

VE Kagan and RM Epand. 2014. Deciphering the misteries of cardiolipins in mitochondria. Chem. Phys. Lipids, 179:1-2.

 

K. DíSouza and RM Epand. 2014. Enrichment of phosphatidylinositols with specific acyl chains. Biochim, Biophys. Acta

1838(6):1501-108.

 

B. Mishra, RF Epand, RM Epand and G. Wang. 2013. Structural location determines functional roles of the basic amino

acids of KR-12, the smallest antimicrobial peptide from human cathelicidin LL-37. RSC Advances, 42(3):19560-19571.

 

YV Shulga, D. Loukov, PT Ivanova, SB Milne, DS Myers, GM Hatch, G. Umeh, D. Jalan, MD Fullerton, GR Steinberg,

MK Topham, HA Brown and RM Epand. 2013. Diacylglycerol kinase delta promotes lipogenesis. Biochemistry

52(44):7766-7776.

 

U. Ros, MA Edwards, RF Epand, ME Lanio, S. Schreier, CM Yip, C. Alvarez and RM Epand. 2013. The Sticholysin

family of pore-forming toxins induces the mixing of lipids in membrane domains. Biochim. Biophys. Acta 1828(11):

2757-2762.

 

AC Brown, NV Balashova, RM Epand, RF Epand, A. Bragin, MC Kachlany, MJ Walters, Y Du, K. Boescze-Battaglia

and ET Lally. 2013. Aggregatibacter actinomycetemcomitans leukotoxin utilizes a cholesterol recognition/aminoacid

consensus site for membrane association. J Biol. Chem. 288(32):23607-23621.

 

K. Goldberg, H. Sarig, F. Zaknoon, RF Epand, RM Epand and A. Mor. 2013. Sensitization of Gram-negative bacteria by

targeting the membrane potential. FASEB J. 27(9):3818-3826.

 

CC Colpitts, AV Ustinov, RF Epand, RM Epand VA Korshun and LM Schang. 2013. 5-(Perylen)-3-yl)sthynyl-arabino-uridine

(aUY11) an arabino-based rigid amphipathic fusion inhibitor, targets virion envelop lipids to inhibit fusion of influenza virus,

hepatitis C virus and other enveloped viruses.J. Virol. 87(7):3640-3654.

 

A. Prodeus, B. Berno, MK Topham and RM Epand. 2013. The basis of the substrate specificity of the epsilon isoform

of human diacylglycerol kinase is not a consequence of competing hydrolysis of ATP. Chem. Phys. Lipids, 166:26-30.

 

U. Schalttner, M. Tokarska-Schlattner, S. Ramirez, YY Tyurina, AA Amoscato, D. Mohammadsanyi, Z. Huang, J. Jiang,

N. Yanamala, A. Seffouh, M. Boissan, RF Epand, RM Epand, J. Klein-Seethamaran, Lacombe ML and VE Kagan. 2013.

Dual function of mitochondrial Nm23-H4 in phosphotransfer and intermembrane lipid transfer: a cardiolipin

dependent-switch. J. Biol. Chem., 288:111-121.

 

YV Shulga, RA Anderson, MK Topham and RM Epand. 2012. Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit

acyl-chain selectivity for both substrate and lipid activator. J. Biol. Chem. 287(43):35953-35963.

 

M. Schlame, D. Acehan, B. Berno, Y. Xu, S. Valvo, M. Ren, DL Stokes and RM Epand. 2012. The physical state of lipid

substrates provides transacylation specificity to tafazzin. Nature Chem. Biol., 8:862-869.

 

M. Tokarska-Schlattner, RF Epand, F. Meiler, G. Zandomeneghi, D. Neumann, HR Widmer, BH Meier, RM Epand, V. Sacks and

U. Schlattner. 2012. Phosphocreatine interacts with phospholipids, affects membrane properties and exerts membrane-protective effects.

PLoS One, 7(8):e43178.

 

P. Wadhwani, RF Epand, N. Heidenreich, J. Burck, AS Ulrich and RM Epand. 2012. Membrane-active peptides and the clustering

of anionic lipids. Biophys. J. 103(2):265-274.

 

F. Zaknoon, K. Goldberg, H. Sarig, RF Epand, RM Epand A. Mor. 2012. Antibacterial properties of an oligo-hexyl-lysyl hexamer

targeting GramĖnegative species. Antimicrob. Agents Chemother. 56(9):4827-4832.

 

G. Wang, RF Epand, VC Thomas, K. Bayles and RM Epand. 2012. Decoding the functional role of cationic side chains of the major

antimicrobial region of hyman cathelicidin LL-37. Antimicrobial Agents and Chemotherapy, 56(2):845-856.

 

AC Brown, K. Boesce-Battaglia, Y. Du, FP Stefano, IR Kieba, RF Epand, L Kabalis, PL Yeagle, RM Epand, ET Lally (2012).

Aggregatibacter Actinomicetemcomitans leukotoxin cytotoxicity occurs through bilayer destabilization. Cell Microbiol., 14(6):869-881.

 

K. DíSouza and RM Epand. 2012. Catalytic activity and acyl-chain selectivity of diacylglycerol kinase-e are modulated by residues in

and near the lipoxygenase-like motif. J. Mol. Biol. 416(5):619-628.

 

RM Epand. 2012. Recognition of polyunsaturated acyl chains by enzymes acting on membrane lipids. B.B.A. Biomembranes, 1818(4):957-962.

 

RM Epand. 2012. Further insights into the properties of the HIV-gp41 fusion domain: commentary on the article by AL Lai et al, J.

Mol. Biol.418(1-2):1-2.

 

S. Aoki and RM Epand. 2012. Caviolin-1 hydrophobic segment peptides insertion into membrane mimetic systems. Role of Proline residue.

B.B.A. Biomembranes, 1818(1):12-18.

 

 

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LINK to:

  Biophysical Society

CSBMCB

American Peptide Society

Protein Society

ASBMB

FASEB

Biophysical Society of Canada